Threshold Ventures is a venture capital firm specializing in early-stage investments in healthcare and enterprise software sectors. Founded in 2019, the firm focuses on Series A and Series B funding rounds, primarily targeting software and software-enabled companies serving customers in healthcare and business. Threshold Ventures is known for its active investment approach, often taking board seats in portfolio companies and maintaining involvement through IPOs and beyond.

The firm has structured its funds to allow continued investment as companies develop, sometimes doubling or tripling investments across multiple rounds. Notable portfolio companies include BetterUp, Doximity, Front, LaunchDarkly, Livongo, Rippling, Remitly, and Talkdesk. The firm's investment team has decades of experience partnering with founders to build high-growth companies that transform industries with innovative solutions.