Threshold Ventures is an early-stage venture capital firm based in Menlo Park, California, specializing in investments in healthcare and enterprise software sectors. The firm primarily focuses on Series A and Series B funding rounds, aiming to partner with visionary founders to bring innovative solutions to market. Threshold Ventures is known for its active involvement in portfolio companies, often taking board seats and supporting them through various growth stages, including IPOs.

The firm's investment philosophy emphasizes a high-conviction approach, concentrating on a select number of investments to provide dedicated support and resources. Notable portfolio companies include Box, Doximity, LaunchDarkly, Livongo, Redfin, Remitly, Talkdesk, and Twilio. Threshold Ventures also runs a podcast called "The Startup Solution," hosted by partner Heidi Roizen, offering entrepreneurial guidance and insights.