The Top Series B Investors & Venture Capital Firms in 2025

Our complete guide in 2025 for the top investors for Series B stage startups.

Contributors: Carl Lager

Last updated: April 21, 2025

Skyscrapers from the bottom with statement in the sky saying 'Series B Investors'Skyscrapers from the bottom with statement in the sky saying 'Series B Investors'

The Changing Landscape of Series B Funding

By 2025, raising a Series B round has become a defining milestone that separates promising early-stage ventures from scalable, fundable businesses. Unlike the peak years of 2021, when capital was abundant and due diligence was lighter, today’s Series B landscape is marked by intense scrutiny. Investors are focused not only on growth metrics but also on underlying business fundamentals.

Startups seeking Series B funding must now present meaningful revenue traction, a clear path to profitability, and a disciplined financial strategy. Venture firms are asking tougher questions—conducting deep dives into customer satisfaction, retention patterns, and gross margin trends. Simply showing potential isn’t enough anymore. Founders need to prove they can build durable, efficient businesses in real time.

Investor Protections and Capital Structure Trends

The shift in expectations has also changed how deals are structured. During the 2022–2023 correction, many Series B rounds included more investor protections—such as liquidation preferences or anti-dilution clauses—to mitigate downside risk. Although these terms began normalizing in late 2023, they left a lasting imprint on how capital is deployed.

Venture debt has also become more prevalent at this stage. For many startups, it’s a tool to extend runway without significant dilution—especially for founders holding out for a stronger valuation in their next equity round.

logos of series b stage venture capital firmslogos of series b stage venture capital firms

Top Venture Capital Firms Leading Series B Deals

Only a select group of Series B venture firms continues to actively deploy capital in today’s cautious environment. The list includes major players like Sequoia Capital, Andreessen Horowitz (a16z), Lightspeed Venture Partners, Insight Partners, and Coatue—all of whom are backing companies that show breakout momentum in sectors like AI, fintech, deep tech, and enterprise infrastructure.

While many hedge funds and crossover investors have scaled back, these core firms are doubling down on businesses with tangible market traction. Their approach has shifted from chasing velocity to investing in clarity: sustainable growth, measurable retention, and operating discipline.

Sector Hotspots for Series B Investment

Notably, certain sectors have become magnets for Series B funding. Artificial intelligence, semiconductor technology, cybersecurity, and robotics are among the most favored verticals. These industries tend to support defensible IP, long-term moats, and global scalability—making them particularly attractive for Series B-stage capital.

Market Correction and the Series B Crunch

The Series B environment has become significantly more competitive. This tightening is part of what many investors now call the "Series B Crunch." The reality is that many startups who raised large Series A rounds during the boom years of 2021–2022 are now struggling to meet investor expectations.

As a result, follow-on funding has slowed, and the median pre-money valuation at Series B dropped to approximately $50 million in 2023—a major correction from the previous cycle’s highs.The fundraising window has also lengthened. Where a Series B might once have followed a Series A within 12 to 18 months, it now often takes 24 months or more. Founders are being forced to do more with less, proving their scalability before the next raise is even on the table.

What Investors Expect in 2025

Today’s Series B investors expect:

  • Evidence of scalable revenue growth

  • Strong customer adoption with healthy retention

  • Efficient burn relative to ARR or GMV

  • A clear roadmap to break-even or profitability

In short, startups must not just be growing—they must be maturing.

What Founders Need to Raise a Series B in 2025

Raising a successful Series B in 2025 starts with realistic planning and performance-driven storytelling. Founders need to demonstrate more than strong metrics—they must show how those numbers translate into long-term defensibility and scale.

Venture capital firms are still writing large checks, but only for businesses with operational discipline, market validation, and clear product-market fit. Those that can show controlled burn, efficient go-to-market strategies, and traction in critical customer segments are still seeing significant investor appetite.

Final Takeaway

The bar has risen—but so has the opportunity. Founders that align with the new expectations aren’t just surviving—they’re thriving, often on stronger footing than those who scaled too quickly in earlier cycles.

All Leading Series B Investors & Venture Capital Firms

Our complete list of all active venture capital firms and investors investing in Series B rounds.

Long Ridge Equity Partners
Founded
2007
Investments
25
Exits
6
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
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LongHash Ventures
Founded
2018
Investments
70
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Longitude Capital
Founded
2006
Investments
103
Exits
33
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Longworth Venture Partners
Founded
1999
Investments
108
Exits
39
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Loup Ventures
Founded
2017
Investments
26
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Lowercase Capital
Founded
2010
Investments
188
Exits
63
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Loyal VC
Founded
2018
Investments
132
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Lumia Capital
Founded
2011
Investments
50
Exits
14
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Lumira Ventures
Founded
1989
Investments
82
Exits
19
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Lunch Partners
Founded
2017
Investments
11
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Lux Capital
Founded
2000
Investments
403
Exits
47
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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M12 - Microsoft's Venture Fund
Founded
2016
Investments
251
Exits
24
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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M25
Founded
2015
Investments
145
Exits
17
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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M3 Ventures
Founded
2015
Investments
38
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MANTIS Venture Capital
Founded
2019
Investments
50
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MATH Venture Partners
Founded
2014
Investments
60
Exits
10
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MB Venture Partners
Founded
2001
Investments
90
Exits
12
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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MDI Ventures
Founded
2016
Investments
82
Exits
10
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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MFV Partners
Founded
2018
Investments
7
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MHS Capital
Founded
2006
Investments
63
Exits
17
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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MMC Ventures
Founded
2000
Investments
180
Exits
23
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MSD Capital
Founded
1998
Investments
44
Exits
14
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MSD Partners
Founded
2009
Investments
14
Exits
3
Investment stage
Series A
Series B
Series C
Company profile
See full breakdown
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MTech Capital
Founded
2016
Investments
20
Exits
1
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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MVM Life Science Partners
Founded
1997
Investments
59
Exits
21
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Madrona Venture Group
Founded
1995
Investments
450
Exits
92
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Magenta Venture Partners
Founded
2018
Investments
8
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Magma Partners
Founded
2014
Investments
98
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Magma Venture Partners
Founded
1999
Investments
115
Exits
24
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Magnetic Ventures
Founded
2019
Investments
13
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Main Sequence Ventures
Founded
2017
Investments
46
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Makers Fund
Founded
2017
Investments
80
Exits
9
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Maki.vc
Founded
2018
Investments
61
Exits
1
Investment stage
Seed
Series A
Series B
Pre-Seed
Company profile
See full breakdown
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ManchesterStory Group
Founded
2017
Investments
30
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Manhattan Venture Partners
Founded
2014
Investments
53
Exits
21
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
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Maniv Mobility
Founded
2014
Investments
64
Exits
5
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Manta Ray Ventures
Founded
2011
Investments
51
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Marathon Venture Capital
Founded
2017
Investments
25
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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March Capital
Founded
2014
Investments
109
Exits
15
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Marker
Founded
2011
Investments
91
Exits
19
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Marlinspike Capital
Founded
2020
Investments
5
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Mass General Brigham Ventures
Founded
2008
Investments
11
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Masthead Venture Partners
Founded
1998
Investments
51
Exits
11
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Mastry
Founded
Investments
1
Exits
0
Investment stage
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Matchstick Ventures
Founded
2015
Investments
100
Exits
9
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Matrix Partners China
Founded
1977
Investments
653
Exits
43
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
View VC Firm
Matrix Partners India
Founded
2006
Investments
178
Exits
12
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Maven Ventures
Founded
2013
Investments
82
Exits
13
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Maverick Capital
Founded
1993
Investments
10
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Mazarine Ventures
Founded
2017
Investments
11
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Measure 8 Venture Partners
Founded
2016
Investments
5
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Mechanism Capital
Founded
2020
Investments
66
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Mendoza Ventures
Founded
2016
Investments
10
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Menlo Ventures
Founded
1976
Investments
717
Exits
167
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Mercato Partners
Founded
2007
Investments
36
Exits
12
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Merida Capital Holdings
Founded
2016
Investments
24
Exits
3
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
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Meridian Street Capital
Founded
2015
Investments
32
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Meritech Capital Partners
Founded
1999
Investments
312
Exits
125
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
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Meron Capital
Founded
2016
Investments
21
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MicroVentures
Founded
2009
Investments
35
Exits
7
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Microsoft for Startups
Founded
2018
Investments
45
Exits
9
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Milano Investment Partners SGR
Founded
2017
Investments
33
Exits
0
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Millennium Technology Value Partners
Founded
2000
Investments
54
Exits
25
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Mindrock Capital
Founded
2018
Investments
29
Exits
5
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
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Mindset Ventures
Founded
2016
Investments
69
Exits
5
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Mithril Capital Management
Founded
2012
Investments
54
Exits
11
Investment stage
Series B
Series C
Series D
Seed
Company profile
See full breakdown
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MizMaa Ventures
Founded
2016
Investments
34
Exits
6
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Mohr Davidow Ventures
Founded
1983
Investments
304
Exits
80
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Molten Ventures
Founded
2006
Investments
188
Exits
44
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Momenta Ventures
Founded
2012
Investments
54
Exits
7
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Monashees
Founded
2005
Investments
213
Exits
25
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Monitor Ventures
Founded
2003
Investments
34
Exits
6
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Monochrome Capital
Founded
2021
Investments
3
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Monogram Capital Partners
Founded
2014
Investments
23
Exits
0
Investment stage
Pre-Seed
Seed
Series A
Series B
Company profile
See full breakdown
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Monozukuri Ventures
Founded
2015
Investments
51
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Monterro
Founded
2012
Investments
18
Exits
0
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
View VC Firm
Moonshots Capital
Founded
2014
Investments
132
Exits
15
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Moore Venture Partners
Founded
2010
Investments
20
Exits
7
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Morado Ventures
Founded
2010
Investments
216
Exits
45
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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MoreVC
Founded
2006
Investments
46
Exits
5
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Morgenthaler Ventures
Founded
1968
Investments
347
Exits
102
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Morningside Group
Founded
1986
Investments
178
Exits
31
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Morningside Venture Investments
Founded
1986
Investments
30
Exits
6
Investment stage
Series A
Series B
Company profile
See full breakdown
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Mouro Capital
Founded
2020
Investments
71
Exits
3
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Moving Capital
Founded
2018
Investments
101
Exits
5
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Mubadala Capital Ventures
Founded
2017
Investments
80
Exits
15
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Mucker Capital
Founded
2011
Investments
210
Exits
17
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Munich Re Ventures
Founded
2014
Investments
53
Exits
5
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Muse Capital
Founded
2016
Investments
38
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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N49P
Founded
2018
Investments
27
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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NFP Ventures
Founded
2018
Investments
26
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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NFT Ventures
Founded
2013
Investments
52
Exits
5
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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NGEN Partners
Founded
2001
Investments
66
Exits
17
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
NGN Capital
Founded
2003
Investments
38
Exits
13
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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NGP Capital
Founded
2005
Investments
163
Exits
46
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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NJTC Venture Fund
Founded
2001
Investments
25
Exits
11
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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NRG Ventures
Founded
2021
Investments
7
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Naples Technology Ventures
Founded
2018
Investments
20
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Narya Capital
Founded
2019
Investments
12
Exits
1
Investment stage
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Navitas Capital
Founded
2009
Investments
52
Exits
8
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
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What do Series B investors look for in a startup?

Investors at this stage seek startups that demonstrate strong traction, a clear path to profitability, and a scalable product-market fit. These companies should also have the operational capabilities to execute their disruptive vision and conquer larger market shares.

How can a startup find the right Series B investors?

Startups should seek investors who offer not just capital but also strategic mentorship, industry connections, and resources tailored to their specific growth challenges. Networking, investor research, and leveraging existing investor relationships are crucial steps in finding the right Series B investors.

What is Series B capital?

Series B funding is a stage of venture capital financing where growth-oriented companies receive investment to scale operations, enhance their products, and expand market reach after showing solid traction and market fit in their early stages.