The Top Series B Investors & Venture Capital Firms in 2025

Our complete guide in 2025 for the top investors for Series B stage startups.

Contributors: Carl Lager

Last updated: April 21, 2025

Skyscrapers from the bottom with statement in the sky saying 'Series B Investors'Skyscrapers from the bottom with statement in the sky saying 'Series B Investors'

The Changing Landscape of Series B Funding

By 2025, raising a Series B round has become a defining milestone that separates promising early-stage ventures from scalable, fundable businesses. Unlike the peak years of 2021, when capital was abundant and due diligence was lighter, today’s Series B landscape is marked by intense scrutiny. Investors are focused not only on growth metrics but also on underlying business fundamentals.

Startups seeking Series B funding must now present meaningful revenue traction, a clear path to profitability, and a disciplined financial strategy. Venture firms are asking tougher questions—conducting deep dives into customer satisfaction, retention patterns, and gross margin trends. Simply showing potential isn’t enough anymore. Founders need to prove they can build durable, efficient businesses in real time.

Investor Protections and Capital Structure Trends

The shift in expectations has also changed how deals are structured. During the 2022–2023 correction, many Series B rounds included more investor protections—such as liquidation preferences or anti-dilution clauses—to mitigate downside risk. Although these terms began normalizing in late 2023, they left a lasting imprint on how capital is deployed.

Venture debt has also become more prevalent at this stage. For many startups, it’s a tool to extend runway without significant dilution—especially for founders holding out for a stronger valuation in their next equity round.

logos of series b stage venture capital firmslogos of series b stage venture capital firms

Top Venture Capital Firms Leading Series B Deals

Only a select group of Series B venture firms continues to actively deploy capital in today’s cautious environment. The list includes major players like Sequoia Capital, Andreessen Horowitz (a16z), Lightspeed Venture Partners, Insight Partners, and Coatue—all of whom are backing companies that show breakout momentum in sectors like AI, fintech, deep tech, and enterprise infrastructure.

While many hedge funds and crossover investors have scaled back, these core firms are doubling down on businesses with tangible market traction. Their approach has shifted from chasing velocity to investing in clarity: sustainable growth, measurable retention, and operating discipline.

Sector Hotspots for Series B Investment

Notably, certain sectors have become magnets for Series B funding. Artificial intelligence, semiconductor technology, cybersecurity, and robotics are among the most favored verticals. These industries tend to support defensible IP, long-term moats, and global scalability—making them particularly attractive for Series B-stage capital.

Market Correction and the Series B Crunch

The Series B environment has become significantly more competitive. This tightening is part of what many investors now call the "Series B Crunch." The reality is that many startups who raised large Series A rounds during the boom years of 2021–2022 are now struggling to meet investor expectations.

As a result, follow-on funding has slowed, and the median pre-money valuation at Series B dropped to approximately $50 million in 2023—a major correction from the previous cycle’s highs.The fundraising window has also lengthened. Where a Series B might once have followed a Series A within 12 to 18 months, it now often takes 24 months or more. Founders are being forced to do more with less, proving their scalability before the next raise is even on the table.

What Investors Expect in 2025

Today’s Series B investors expect:

  • Evidence of scalable revenue growth

  • Strong customer adoption with healthy retention

  • Efficient burn relative to ARR or GMV

  • A clear roadmap to break-even or profitability

In short, startups must not just be growing—they must be maturing.

What Founders Need to Raise a Series B in 2025

Raising a successful Series B in 2025 starts with realistic planning and performance-driven storytelling. Founders need to demonstrate more than strong metrics—they must show how those numbers translate into long-term defensibility and scale.

Venture capital firms are still writing large checks, but only for businesses with operational discipline, market validation, and clear product-market fit. Those that can show controlled burn, efficient go-to-market strategies, and traction in critical customer segments are still seeing significant investor appetite.

Final Takeaway

The bar has risen—but so has the opportunity. Founders that align with the new expectations aren’t just surviving—they’re thriving, often on stronger footing than those who scaled too quickly in earlier cycles.

All Leading Series B Investors & Venture Capital Firms

Our complete list of all active venture capital firms and investors investing in Series B rounds.

Supply Chain Ventures
Founded
2001
Investments
15
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Susquehanna Growth Equity
Founded
2006
Investments
91
Exits
29
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
View VC Firm
Susquehanna International Group (SIG)
Founded
1987
Investments
106
Exits
8
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Sutter Hill Ventures
Founded
1964
Investments
312
Exits
94
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Sutton Capital
Founded
2020
Investments
10
Exits
1
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Swiftarc Ventures
Founded
2019
Investments
8
Exits
0
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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SymphonyAI
Founded
2017
Investments
5
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TA Ventures
Founded
2010
Investments
166
Exits
38
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TCG
Founded
2009
Investments
92
Exits
21
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TCP Venture Capital
Founded
2012
Investments
45
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TDF Ventures
Founded
2004
Investments
106
Exits
26
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TEDCO
Founded
1998
Investments
110
Exits
13
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TFX Capital
Founded
2015
Investments
1
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TL Ventures
Founded
1988
Investments
116
Exits
35
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TLV Partners
Founded
2015
Investments
79
Exits
6
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TPG Biotech
Founded
2002
Investments
71
Exits
22
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TPG Growth
Founded
2007
Investments
116
Exits
48
Investment stage
Series B
Series C
Series D
Series A
Company profile
See full breakdown
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TRGC
Founded
2018
Investments
33
Exits
0
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TSVC
Founded
2010
Investments
230
Exits
43
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tal Ventures
Founded
2021
Investments
23
Exits
0
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Talis Capital
Founded
2009
Investments
70
Exits
11
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tallwood Venture Capital
Founded
2000
Investments
56
Exits
15
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tamarack Global
Founded
2018
Investments
7
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TampaBay.Ventures
Founded
2021
Investments
4
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tapas Capital
Founded
2017
Investments
6
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Target Global
Founded
2012
Investments
181
Exits
19
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Team Launch
Founded
2014
Investments
7
Exits
1
Investment stage
Series B
Company profile
See full breakdown
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Team8
Founded
2014
Investments
28
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Teamworthy Ventures
Founded
2014
Investments
52
Exits
8
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TechFarms Capital
Founded
2019
Investments
2
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Techstars Anywhere Accelerator
Founded
2017
Investments
50
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Teknasyon
Founded
2013
Investments
19
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TeleSoft Partners
Founded
1996
Investments
92
Exits
28
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Telegraph Hill Capital
Founded
2007
Investments
56
Exits
10
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Telegraph Hill Partners
Founded
2001
Investments
36
Exits
12
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Telstra Ventures
Founded
2011
Investments
111
Exits
25
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Ten Eleven Ventures
Founded
2014
Investments
58
Exits
12
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TenOneTen Ventures
Founded
2011
Investments
130
Exits
21
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tenaya Capital
Founded
2009
Investments
184
Exits
64
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tenzing Capital (Tenzing.vc)
Founded
2021
Investments
4
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Terra Venture Partners
Founded
2006
Investments
33
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tesi
Founded
1995
Investments
128
Exits
16
Investment stage
Series A
Series B
Series C
Series D
Company profile
See full breakdown
View VC Firm
Thayer Ventures
Founded
2007
Investments
45
Exits
4
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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The Alchemy Fund
Founded
1997
Investments
4
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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The Argentum Group
Founded
1990
Investments
40
Exits
11
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
The Aurora Funds
Founded
1994
Investments
71
Exits
19
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
The Column Group
Founded
2006
Investments
99
Exits
18
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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The E14 Fund
Founded
2013
Investments
60
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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The Family
Founded
2013
Investments
73
Exits
9
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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The Group Ventures
Founded
2018
Investments
8
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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The Venture Reality Fund/VRF
Founded
2016
Investments
49
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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The Vertical Group
Founded
1988
Investments
64
Exits
20
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
View VC Firm
The Westly Group
Founded
2007
Investments
92
Exits
19
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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The Yield Lab
Founded
2014
Investments
49
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Third Point Ventures
Founded
1995
Investments
96
Exits
26
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
View VC Firm
Third Rock Ventures
Founded
2007
Investments
123
Exits
34
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Thomas, McNerney & Partners
Founded
2003
Investments
48
Exits
17
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Thomvest Ventures
Founded
1996
Investments
120
Exits
28
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Threshold
Founded
2019
Investments
877
Exits
199
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Thrill Impact Ventures
Founded
2019
Investments
13
Exits
2
Investment stage
Series A
Series B
Company profile
See full breakdown
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Thrive Capital
Founded
2009
Investments
302
Exits
63
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Thursday Ventures
Founded
2017
Investments
18
Exits
0
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tidemark Capital
Founded
2021
Investments
3
Exits
0
Investment stage
Series B
Series C
Series D
Company profile
See full breakdown
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Tiger Global Management
Founded
2001
Investments
1058
Exits
129
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tiller Partners
Founded
2012
Investments
22
Exits
7
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tippet Venture Partners
Founded
Investments
6
Exits
0
Investment stage
Series B
Company profile
See full breakdown
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Titan Capital
Founded
2015
Investments
102
Exits
1
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Toba Capital
Founded
2012
Investments
111
Exits
24
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tomahawk.VC
Founded
2020
Investments
15
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Top Tier Capital Partners
Founded
1999
Investments
52
Exits
17
Investment stage
Series C
Series D
Series A
Series B
Company profile
See full breakdown
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Topmark Partners
Founded
2013
Investments
22
Exits
6
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Topspin Partners
Founded
2000
Investments
51
Exits
15
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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TotalEnergies Ventures
Founded
2008
Investments
86
Exits
13
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Touchdown Ventures
Founded
2014
Investments
64
Exits
12
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Translink Capital
Founded
2007
Investments
139
Exits
28
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tri-Valley Ventures
Founded
2017
Investments
9
Exits
2
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Triangle Peak Partners
Founded
2008
Investments
73
Exits
24
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Tribe Capital
Founded
2018
Investments
145
Exits
2
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
Tribeca Early Stage Partners
Founded
2014
Investments
38
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Triblock
Founded
2019
Investments
22
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Trind Ventures
Founded
1999
Investments
19
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Trinity Ventures
Founded
1986
Investments
464
Exits
140
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Triventures
Founded
2008
Investments
41
Exits
6
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Trolley Venture Partners
Founded
2017
Investments
8
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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True Ventures
Founded
2005
Investments
621
Exits
105
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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True.
Founded
2013
Investments
64
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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TrueSight Ventures
Founded
2018
Investments
52
Exits
3
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tsing Capital
Founded
2001
Investments
32
Exits
3
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Tuesday Capital
Founded
2011
Investments
299
Exits
95
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Tullis Health Investors
Founded
1986
Investments
41
Exits
16
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Two Bear Capital
Founded
2019
Investments
10
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
View VC Firm
Tyche Partners
Founded
2015
Investments
30
Exits
3
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
View VC Firm
U.S. Venture Partners
Founded
1981
Investments
559
Exits
156
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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UBS
Founded
1854
Investments
71
Exits
17
Investment stage
Series C
Series D
Series B
Company profile
See full breakdown
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UP Partners
Founded
2020
Investments
14
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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US Venture Partners
Founded
1981
Investments
557
Exits
155
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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USAA
Founded
1922
Investments
41
Exits
13
Investment stage
Seed
Series A
Series B
Series C
Series D
Company profile
See full breakdown
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Unanimous Capital
Founded
2018
Investments
10
Exits
0
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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Uncork Capital
Founded
2004
Investments
386
Exits
98
Investment stage
Pre-Seed
Seed
Series A
Series B
Series C
Company profile
See full breakdown
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Unicorn India Ventures
Founded
2015
Investments
43
Exits
4
Investment stage
Seed
Series A
Series B
Company profile
See full breakdown
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What do Series B investors look for in a startup?

Investors at this stage seek startups that demonstrate strong traction, a clear path to profitability, and a scalable product-market fit. These companies should also have the operational capabilities to execute their disruptive vision and conquer larger market shares.

How can a startup find the right Series B investors?

Startups should seek investors who offer not just capital but also strategic mentorship, industry connections, and resources tailored to their specific growth challenges. Networking, investor research, and leveraging existing investor relationships are crucial steps in finding the right Series B investors.

What is Series B capital?

Series B funding is a stage of venture capital financing where growth-oriented companies receive investment to scale operations, enhance their products, and expand market reach after showing solid traction and market fit in their early stages.